aat bioquest quest graph ld 50 /ed 50 calculator  (AAT Bioquest)

Journal: bioRxiv

Article Title: Strong SARS-CoV-2 N-specific CD8 + T immunity induced by engineered extracellular vesicles associates with protection from lethal infection in mice

doi: 10.1101/2022.01.10.475620

Figure Lengend Snippet: Antiviral effect induced by high levels of N-specific CD8 + T cell immunity. ( A ) CD8 + T cell immune response in C57 Bl/6 K18-hACE-2 mice injected with vectors expressing either Nef mut /S1, Nef mut /N or, as control, Nef mut alone. PBMCs were isolated by retro orbital bleeding and, after erythrocyte lysis, were incubated o.n. with or without 5 μg/mL of either unrelated or SARS-CoV-2 related peptides in triplicate IFN-γ EliSpot microwells. Shown are the number of SFUs/10 5 PBMCs as mean values of triplicates after subtraction of values from wells treated with an unrelated peptide. Intragroup mean values + SD are reported. ( B ) Kaplan–Meier survival curve calculated for groups of C57 Bl/6 K18-hACE-2 mice infected with 4.4 LD 50 of SARS-CoV-2. Differences between Kaplan-Meier survival curves relative to S1- and N-immunized groups of mice were statistically significant (log-rank test, p = 0.01285). ( C ) Relative weight loss in each injected mice after SARS-CoV-2 challenge. Identification numbers of each mouse are reported on the right of each panel. SFUs/10 5 PBMCs for each low and high responder N-immunized mouse are also indicated together with intergroup mean values. Shown are cumulative data from two experiments.

Article Snippet: For the in vivo titration, virus dilutions and number of deaths/group after challenge were used to calculate the LD 50 (Quest Graph™ LD 50 Calculator, AAT Bioquest, Inc.).

Techniques: Injection, Expressing, Isolation, Lysis, Incubation, Enzyme-linked Immunospot, Infection

Journal: bioRxiv

Article Title: Strong SARS-CoV-2 N-specific CD8 + T immunity induced by engineered extracellular vesicles associates with protection from lethal infection in mice

doi: 10.1101/2022.01.10.475620

Figure Lengend Snippet: Relative weight loss in mice injected with 10 μg each of Nef mut /S1, Nef mut /S2 and Nef mut /N expressing vectors, and then infected with 5 LD 50 of SARS-CoV-2. Identification numbers for each mouse are reported on the right together with the counts of SFUs/10 5 PBMCs after incubation with the N 219-228 peptide. S2-specific SFU counts as measured using a pool of S2 peptides were similar in each mouse.

Article Snippet: For the in vivo titration, virus dilutions and number of deaths/group after challenge were used to calculate the LD 50 (Quest Graph™ LD 50 Calculator, AAT Bioquest, Inc.).

Techniques: Injection, Expressing, Infection, Incubation